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ONLINEISSN:1347-6947
PRINTISSN:0916-8451
Bioscience, Biotechnology, and Biochemistry
Vol. 63 (1999) , No. 4 pp.632-637
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Two Cytosolic Cyclophilin Genes of Arabidopsis thaliana Differently Regulated in Temporal- and Organ-Specific Expression
Takeshi SAITO1), Kei TADAKUMA1), Naoko TAKAHASHI1), Hiroyuki ASHIDA1), Katsunori TANAKA2), Makoto KAWAMUKAI2), Hideyuki MATSUDA2) and Tsuyoshi NAKAGAWA1)
1) Research Institute of Molecular Genetics, Shimane University
2) Department of Applied Bioscience and Biotechnology, Fuculty of Life and Environmental Science, Shimane University
(Received September 1, 1998)
(Accepted December 4, 1998)
  We have previously isolated two closely related genes (ATCYP1 and ATCYP2) each encoding a cytosolic cyclophilin of Arabidopsis thaliana. Here we tested expression patterns of these two genes by Northern analysis and by histochemical analysis with transgenic plants carrying the promoter: β-glucuronidase (GUS) fusion. The results showed that ATCYP1 is predominantly transcribed in vascular tissue and flowers, but ATCYP2 is at higher levels in younger leaves. The different expression patterns seemed to be conferred by the quite different promoter structures carrying various cis elements. Our finding suggests that the two cyclophilins have different roles in Arabidopsis thaliana cells.
Key words:Arabidopsis thaliana; β-glucuronidase; cyclophilin; vascular tissue

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To cite this article:
Takeshi SAITO, Kei TADAKUMA, Naoko TAKAHASHI, Hiroyuki ASHIDA, Katsunori TANAKA, Makoto KAWAMUKAI, Hideyuki MATSUDA and Tsuyoshi NAKAGAWA, “Two Cytosolic Cyclophilin Genes of Arabidopsis thaliana Differently Regulated in Temporal- and Organ-Specific Expression”, Biosci. Biotechnol. Biochem., Vol. 63, 632-637 (1999) .

doi:10.1271/bbb.63.632
JOI  JST.JSTAGE/bbb/63.632
Copyright (c) 2005 by Japan Society for Bioscience, Biotechnology, and Agrochemistry



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