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| | ONLINE | ISSN | : | 1347-6947 | | PRINT | ISSN | : | 0916-8451 |
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| | Bioscience, Biotechnology, and Biochemistry |
| Vol. 63 (1999) , No. 8 pp.1346-1352 |
| [Image PDF (3112K)] [References]  |
 | XynX, a Possible Exo-xylanase of Aeromonas caviae ME-1 that Produces Exclusively Xylobiose and Xylotetraose from Xylan
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| | 1) Department of Biotechnology, Faculty of Agriculture, Gifu University
2) Molecular Genetics Research Center, Gifu University |
| (Received January 20, 1999)
(Accepted April 16, 1999)
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| | A gene, xynX, encoding a novel xylanase, was cloned from Aeromonas caviae ME-1. This gene encoded an enzyme that was constituted of 334 amino acid residues (38,580 Da) and was similar in sequence to Family 10 (Family F) β-1,4 endo-xylanases. XynX produced only xylobiose and xylotetraose from birch wood xylan, and xylotriose, xylopentaose, and higher oligosaccharides were not detected in the TLC analysis. We designated it as X2/X4-forming xylanase. This enzyme does not have transglycosylation activity. These data suggested that this enzyme is a possible exo-xylanase. According to homology modeling, the enzyme has a ring-shaped (α/β)8 barrel (TIM barrel) structure, typical of Family 10 endo-xylanases, with the extraordinary feature of a longer bottom-loop structure.
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| To cite this article: | | Kengo USUI, Keiji IBATA, Tohru SUZUKI and Keiichi KAWAI, “XynX, a Possible Exo-xylanase of Aeromonas caviae ME-1 that Produces Exclusively Xylobiose and Xylotetraose from Xylan”, Biosci. Biotechnol. Biochem., Vol. 63, 1346-1352 (1999) . | |
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| doi:10.1271/bbb.63.1346 | | JOI JST.JSTAGE/bbb/63.1346 |
| Copyright (c) 2005 by Japan Society for Bioscience, Biotechnology, and Agrochemistry |
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