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| | ONLINE | ISSN | : | 1347-6947 | | PRINT | ISSN | : | 0916-8451 |
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| | Bioscience, Biotechnology, and Biochemistry |
| Vol. 64 (2000) , No. 6 pp.1133-1141 |
| [Image PDF (1324K)] [References]  |
 | A New High-alkaline and High-molecular-weight Pectate Lyase from a Bacillus Isolate: Enzymatic Properties and Cloning of the Gene for the Enzyme
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| | | 1) Tochigi Research Laboratories of Kao Corporation |
| (Received October 14, 1999)
(Accepted January 26, 2000)
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| | A pectate lyase (Pel; pectate transeliminase: EC4.2.2.2.), designated Pel-15H, was found in an alkaline culture of Bacillus sp. strain KSM-P15 and purified to homogeneity by sequential column chromatographies. The molecular weight of the enzyme determined by SDS-polyacrylamide gel electrophoresis was approximately 70,000 and the pI was around pH 4.6. Pel-15H randomly trans-eliminated polygalacturonate in the presence of Ca2+ ions, and the maximum activity was observed at pH 11.5 and at 55°C in glycine-NaOH buffer. The gene for Pel-15H was cloned and sequenced, and the structural gene contained a 2,031-bp open reading frame that encoded 677 amino acids including a possible 28-amino-acid signal sequence. The mature enzyme (649 amino acids, molecular weight 69,550) showed very low similarity to Pels from Bacillus with 12.7-18.2% identity. Interestingly, part of the amino acid sequence of Pel-15H had fairly high similarity only to an N-terminal half of PelL and a C-terminal half of PelX from Erwinia chrysanthemi 3937, and a C-terminal half of PelX from E. chrysanthemi EC16 (approximately 35% identity for all).
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| To cite this article: | | Akinori OGAWA, Kazuhisa SAWADA, Kazuhiro SAITO, Yoshihiro HAKAMADA, Nobuyuki SUMITOMO, Yuji HATADA, Tohru KOBAYASHI and Susumu ITO, “A New High-alkaline and High-molecular-weight Pectate Lyase from a Bacillus Isolate: Enzymatic Properties and Cloning of the Gene for the Enzyme”, Biosci. Biotechnol. Biochem., Vol. 64, 1133-1141 (2000) . | |
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| doi:10.1271/bbb.64.1133 | | JOI JST.JSTAGE/bbb/64.1133 |
| Copyright (c) 2005 by Japan Society for Bioscience, Biotechnology, and Agrochemistry |
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