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| | ONLINE | ISSN | : | 1347-6947 | | PRINT | ISSN | : | 0916-8451 |
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| | Bioscience, Biotechnology, and Biochemistry |
| Vol. 64 (2000) , No. 7 pp.1526-1530 |
| [Image PDF (1416K)] [References]  |
 | Cloning and Characterization of Bovine Stearoyl CoA Desaturase1 cDNA from Adipose Tissues
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| | 1) Department of Animal Science & Technology, School of Agricultural Biotechnology, College of Agriculture & Life Science, Seoul National University
2) Department of Biochemistry, College of Medicine, University of Saskatchewan
3) Department of Genetic Engineering and Institute of Biotechnology, Chonnam National University |
| (Received December 13, 1999)
(Accepted February 24, 2000)
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| | Two overlapping cDNA clones have been isolated from bovine adipose tissue by the reverse-transcription-polymerase chain reaction (RT-PCR) method. The combined sequence of the two clones was 1039 bp in length and encoded 345 amino acids. The deduced amino acid sequence of the clones showed 96.5% similarity to that of sheep SCD and more than 88% similarities to other mammalian SCD1s, indicating that the clones are the cDNAs for the bovine SCD1. The transcript size of the bovine SCD1 was about 4.9 kb, the message was detected in the bovine adipose tissues but not in the liver. Female cattle expressed threefold higher levels of SCD1 mRNA than male animals.
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| To cite this article: | | Myungil CHUNG, Seckho HA, Soonseog JEONG, Jinduck BOK, Kwangkeun CHO, Myunggi BAIK and Yunjaie CHOI, “Cloning and Characterization of Bovine Stearoyl CoA Desaturase1 cDNA from Adipose Tissues”, Biosci. Biotechnol. Biochem., Vol. 64, 1526-1530 (2000) . | |
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| doi:10.1271/bbb.64.1526 | | JOI JST.JSTAGE/bbb/64.1526 |
| Copyright (c) 2005 by Japan Society for Bioscience, Biotechnology, and Agrochemistry |
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