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ONLINEISSN:1347-6947
PRINTISSN:0916-8451
Bioscience, Biotechnology, and Biochemistry
Vol. 65 (2001) , No. 4 pp.977-981
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Cloning and Characterization of a Chitosanase Gene from the Koji Mold Aspergillus oryzae Strain IAM 2660
Xiao-Yong ZHANG1), An-Lan DAI1), Kouji KUROIWA1), Ritsuko KODAIRA1), Masahiro NOGAWA1), Makoto SHIMOSAKA1) and Mitsuo OKAZAKI2)
1) Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
2) Gene Research Center, Shinshu University
(Received November 6, 2000)
(Accepted December 13, 2000)
  A genomic copy of the gene coding for chitosanase (csnA) was isolated from Aspergillus oryzae IAM 2660. A. oryzae csnA contains an open reading frame that encodes a polypeptide of 245 amino acids with a calculated molecular mass of 26,500 Da. The deduced amino acid sequence of A. oryzae csnA indicates extensive similarities to those of other fungal chitosanases.
Key words:chitosanase; Aspergillus; gene cloning; koji mold

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To cite this article:
Xiao-Yong ZHANG, An-Lan DAI, Kouji KUROIWA, Ritsuko KODAIRA, Masahiro NOGAWA, Makoto SHIMOSAKA and Mitsuo OKAZAKI, “Cloning and Characterization of a Chitosanase Gene from the Koji Mold Aspergillus oryzae Strain IAM 2660”, Biosci. Biotechnol. Biochem., Vol. 65, 977-981 (2001) .
doi:10.1271/bbb.65.977
JOI  JST.JSTAGE/bbb/65.977
Copyright (c) 2001 by Japan Society for Bioscience, Biotechnology, and Agrochemistry

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