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| | ONLINE | ISSN | : | 1347-6947 | | PRINT | ISSN | : | 0916-8451 |
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| | Bioscience, Biotechnology, and Biochemistry |
| Vol. 65 (2001) , No. 6 pp.1406-1411 |
| [Image PDF (1089K)] [References]  |
 | Molecular Cloning and Characterization of cel2 from the Fungus Cochlibolus carbonum
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| | 1) Department of Forest Resource, Konkuk University
2) DOE-Plant Research Lab, Michigan State University
3) School of Agricultural Biotechnology, Seoul National University |
| (Received November 27, 2000)
(Accepted January 30, 2001)
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| | A new cellulase gene, cel2, from the filamentous fungus Cochliobolus carbonum was cloned by using egl-1 of Trichoderma reesei as a heterologous probe. DNA blot analysis of cel2 showed that this gene is present as a single copy. The gene contains one 49-bp- intron. cel2 encodes a predicted protein (Cel2p) of 423 amino acids with a molecular mass of 45.8 kDa. The predicted pI is 4.96. It shows similarity to other endoglucanases from various fungi. From the comparison with other cellulase genes, cel2 belongs to family 7 of glucohydrolases. cel2 is located on a 2.5-Mb chromosome in C. carbonum and its expression is repressed by sucrose. A cel2 mutant of C. carbonum was created by transformation-mediated gene disruption. The pathogenicity of the mutant was indistinguishable from the wild type, indicating that cel2 by itself is not important for pathogenicity. |
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| To cite this article: | | Joong-Hoon AHN, Paola SPOSATO, Su-Il KIM and Jonathan D. WALTON, “Molecular Cloning and Characterization of cel2 from the Fungus Cochlibolus carbonum”, Biosci. Biotechnol. Biochem., Vol. 65, 1406-1411 (2001) . | |
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| doi:10.1271/bbb.65.1406 | | JOI JST.JSTAGE/bbb/65.1406 |
| Copyright (c) 2001 by Japan Society for Bioscience, Biotechnology, and Agrochemistry |
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