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ONLINEISSN:1347-6947
PRINTISSN:0916-8451
Bioscience, Biotechnology, and Biochemistry
Vol. 65 (2001) , No. 6 pp.1419-1423
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Trehalose-producing Operon treYZ from Arthrobacter ramosus S34
Takuo YAMAMOTO1), Kazuhiko MARUTA1), Hikaru WATANABE1), Hiroshi YAMASHITA1), Michio KUBOTA1), Shigeharu FUKUDA1) and Masashi KURIMOTO1)
1) Hayashibara Biochemical Laboratories, Inc.
(Received December 11, 2000)
(Accepted January 26, 2001)
  Arthrobacter ramosus S34, which produces trehalose from maltooligosaccharide, was isolated. A trehalose-producing operon, treYZ, was cloned from the genome. Expression experiments with treY and treZ confirmed that they coded malto-oligosyltrehalose synthase and malto-oligosyltrehalose trehalohydrolase, respectively. The amino acid sequence of TreY from A. ramosus S34 and that from Arthrobacter sp. Q36 did not show high identity, nor did those to TreZ.
Key words:trehalose; gene cloning; nucleotide sequence; operon; Arthrobacter

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To cite this article:
Takuo YAMAMOTO, Kazuhiko MARUTA, Hikaru WATANABE, Hiroshi YAMASHITA, Michio KUBOTA, Shigeharu FUKUDA and Masashi KURIMOTO, “Trehalose-producing Operon treYZ from Arthrobacter ramosus S34”, Biosci. Biotechnol. Biochem., Vol. 65, 1419-1423 (2001) .
doi:10.1271/bbb.65.1419
JOI  JST.JSTAGE/bbb/65.1419
Copyright (c) 2001 by Japan Society for Bioscience, Biotechnology, and Agrochemistry

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