TOP > Available Issues > Table of Contents > Abstract
| | ONLINE | ISSN | : | 1347-6947 | | PRINT | ISSN | : | 0916-8451 |
|
| | Bioscience, Biotechnology, and Biochemistry |
| Vol. 65 (2001) , No. 7 pp.1684-1687 |
| [Image PDF (720K)] [References]  |
 | Cloning and Nucleotide Sequence of the Mycodextranase Gene from Streptomyces sp. J-13-3
|
| | 1) Department of Life Sciences Faculty of Agriculture, Kagawa University
2) Department of Biochemistry and Food Science, Faculty of Agriculture, Kagawa University |
| (Received February 9, 2001)
(Accepted March 13, 2001)
|
| | Mycodextranase (EC 3.2.1.61) is an α-glucanase that cleaves α-1,4-bonds of alternating α-1,3- and α-1,4-linked D-glucan (nigeran). The gene encoding mycodextranase from Streptomyces sp. J-13-3 was cloned by hybridization with a degenerate oligonucleotide probe from the amino-terminal amino acid sequence of the enzyme and its nucleotide structure was analyzed. The open reading frame consisted of 1,803 base pairs encoding a signal peptide of 60 amino acids and a mature protein of 540 amino acids with a calculated molecular weight of 56,078. The deduced amino acid sequence showed weak similality to a chitinase homolog from Streptomyces lividans and a chitinase from Xanthomonas sp. |
| | | |
| To cite this article: | | Katsuichiro OKAZAKI, Takashi AMANO, Tetsuhiro MORIMOTO, Takahiro IEMOTO, Toshiyuki KAWABATA, Shigeru HAYAKAWA and Kazuya AKIMITSU, “Cloning and Nucleotide Sequence of the Mycodextranase Gene from Streptomyces sp. J-13-3”, Biosci. Biotechnol. Biochem., Vol. 65, 1684-1687 (2001) . | |
|
| doi:10.1271/bbb.65.1684 | | JOI JST.JSTAGE/bbb/65.1684 |
| Copyright (c) 2001 by Japan Society for Bioscience, Biotechnology, and Agrochemistry |
|
|
