TOP > Available Issues > Table of Contents > Abstract
| | ONLINE | ISSN | : | 1347-6947 | | PRINT | ISSN | : | 0916-8451 |
|
| | Bioscience, Biotechnology, and Biochemistry |
| Vol. 66 (2002) , No. 6 pp.1246-1255 |
| [PDF (1167K)] [References]  |
 | Cloning and Structural Analysis of bglM Gene Coding for the Fungal Cell Wall-lytic β-1,3-Glucan-hydrolase BglM of Bacillus circulans IAM1165
|
| | | 1) Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology |
| (Received October 25, 2001)
(Accepted January 10, 2002)
|
| | Bacillus circulans IAM1165 produces isoforms of β-1,3-glucan-hydrolases. Of these enzymes, the 42-kDa enzyme BglM degrades Aspergillus oryzae cell walls the most actively. A gene coding for a BglM precursor consisting of 411 amino acid residues was cloned. The 27 N-terminal amino acid sequence of the precursor is a signal peptide. The 141 C-terminal amino acid sequence showed a motif of carbohydrate-binding module family 13. This domain bound to pachyman, lichenan, and A. oryzae cell walls. The central domain showed a bacterial β-1,3-glucan-hydrolase motif belonging to glycosyl hydrolase family 16. By removal of the C-terminal domain in the IAM1165 culture, mature BglM was processed to several 27-kDa fragments that hydrolyze a soluble β-1,3-glucan.
|
| | | |
| To cite this article: | | Takeshi ASANO, Jiro TAKI, Mami YAMAMOTO and Rikizo AONO, “Cloning and Structural Analysis of bglM Gene Coding for the Fungal Cell Wall-lytic β-1,3-Glucan-hydrolase BglM of Bacillus circulans IAM1165”, Biosci. Biotechnol. Biochem., Vol. 66, 1246-1255 (2002) . | |
|
| doi:10.1271/bbb.66.1246 | | JOI JST.JSTAGE/bbb/66.1246 |
| Copyright (c) 2003 by Japan Society for Bioscience, Biotechnology, and Agrochemistry |
|
|
