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| | ONLINE | ISSN | : | 1347-6947 | | PRINT | ISSN | : | 0916-8451 |
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| | Bioscience, Biotechnology, and Biochemistry |
| Vol. 68 (2004) , No. 3 pp.739-742 |
| [PDF (85K)] [References]  |
 | Genetic Analysis of an Incomplete bio Operon in a Biotin Auxotrophic Strain of Bacillus subtilis Natto OK2
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| | 1) Laboratory of Molecular Microbiology, College of Agriculture, Ibaraki University
2) Laboratory of Molecular Genetics, College of Science, Rikkyo (St. Paul’s) University |
| (Received August 13, 2003)
(Accepted November 8, 2003)
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| | We describe the genetic analysis of the bio operon of the biotin auxotrophic Bacillus subtilis natto OK2 strain. The OK2 strain would only cross-feed with the Escherichia coli bioB mutant and also grew well in medium containing dethiobiotin. Sequencing analysis revealed two significant genetic alterations in the bioW and bioF genes within the bio operon of the OK2 strain. Complementation analysis with B. subtilis 168 bio mutants demonstrated that only the bioB gene could complement, but other bio operon genes could not. A bio+ transformant, isolated from an OK2 strain, has biotin autotrophy. |
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| To cite this article: | | Mayumi SASAKI, Fujio KAWAMURA and Yasurou KURUSU, “Genetic Analysis of an Incomplete bio Operon in a Biotin Auxotrophic Strain of Bacillus subtilis Natto OK2”, Biosci. Biotechnol. Biochem., Vol. 68, 739-742 (2004) . | |
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| doi:10.1271/bbb.68.739 | | JOI JST.JSTAGE/bbb/68.739 |
| Copyright (c) 2004 by Japan Society for Bioscience, Biotechnology, and Agrochemistry |
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