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| | ONLINE | ISSN | : | 1347-6947 | | PRINT | ISSN | : | 0916-8451 |
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| | Bioscience, Biotechnology, and Biochemistry |
| Vol. 68 (2004) , No. 4 pp.924-926 |
| [PDF (83K)] [References]  |
 | Interaction between a Type-II Dockerin Domain and a Type-II Cohesin Domain from Clostridium thermocellum Cellulosome
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| | 1) Faculty of Bioresources, Mie University
2) Special Research Laboratory II, Toyota Central R&D Laboratories
3) Department of Biotechnology, Pasteur Institute |
| (Received September 24, 2003)
(Accepted November 21, 2003)
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| | The interaction between the type-II dockerin domain of the scaffoldin protein CipA and the type-II cohesin domain of the outer layer protein SdbA is the fundamental mechanism for anchoring the cellulosome to the cell surface of Clostridium thermocellum. We constructed and purified a dockerin polypeptide and a cohesin polypeptide, and determined affinity constants of the interaction between them by the surface plasmon resonance method. The dissociation constant (KD) value was 1.8×10−9 M, which is a little larger than that for the combination of a type-I dockerin and a type-I cohesin. |
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| To cite this article: | | Sadanari JINDOU, Tsutomu KAJINO, Minoru INAGAKI, Shuichi KARITA, Pierre BEGUIN, Tetsuya KIMURA, Kazuo SAKKA and Kunio OHMIYA, “Interaction between a Type-II Dockerin Domain and a Type-II Cohesin Domain from Clostridium thermocellum Cellulosome”, Biosci. Biotechnol. Biochem., Vol. 68, 924-926 (2004) . | |
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| doi:10.1271/bbb.68.924 | | JOI JST.JSTAGE/bbb/68.924 |
| Copyright (c) 2004 by Japan Society for Bioscience, Biotechnology, and Agrochemistry |
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