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| | ONLINE | ISSN | : | 1348-0634 | | PRINT | ISSN | : | 0009-2673 |
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| | Bulletin of the Chemical Society of Japan |
| Vol. 78 (2005) , No. 7 pp.1291-1295 |
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 | Metal Ion-Induced Hetero-Block α-Helical Coiled Coil
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| | | 1) Department of Applied Molecular Chemistry, College of Industrial Technology, Nihon University |
| (Received September 2, 2004)
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To construct polypeptides that undergo a hetero-block α-helical coiled coil structure change due to metal ion-induced self-assembly formation, we designed two kinds of 18-residue polypeptides, Pep3 and Pep4, each with a metal binding site. Pep3 and Pep4 were designed based on the sequence of the isoleucine zipper polypeptide forming the triple-stranded α-helical coiled coils. While Pep3 and Pep4 are too short to form a parallel triple stranded α-helical coiled coil individually, they form the hetero-block α-helical coiled coil structure, (Pep3)3–Ni2+–(Pep4)3 in the presence of the Ni2+ ions. Circular dichroism spectroscopy and size-exclusion gel-filtration chromatography analyses revealed the formation of the (Pep3)3–Mn+–(Pep4)3 hetero-block α-helical coiled coil complex. |
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| doi:10.1246/bcsj.78.1291 | | JOI JST.JSTAGE/bcsj/78.1291 |
| Copyright (c) 2005 The Chemical Society of Japan |
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