BPB : Vol. 29 (2006) , No. 12 2451

Genome Wide Expression Analysis of White Blood Cells and Liver of Pre-diabetic Otsuka Long-Evans Tokushima Fatty (OLETF) Rats Using a cDNA Microarray

Shinya Iida¹⁾, Yuichiro Sato¹⁾, Akihiro Nakaya²⁾, Yasuo Shinohara³⁾, Yasuhiro Hayashi¹⁾, Akihiro Sawada⁴⁾, Hideya Nagata⁵⁾, Noritada Kaji⁴⁾, Hiroyuki Kamiya¹⁾, Yoshinobu Baba⁴⁾ and Hideyoshi Harashima¹⁾

1) Laboratory for Molecular Design of Pharmaceutics, Graduate School of Pharmaceutical Sciences, Hokkaido University
2) Department of Computational Biology, University of Tokyo
3) Institute of Genome Research, The University of Tokushima
4) Department of Applied Chemistry, Graduate School of Engineering, Nagoya University
5) Health Technology Research Center, National Institute of Advanced Industrial Sciences and Technology (AIST)

(Received June 30, 2006)
(Accepted September 11, 2006)

In a prior study, we reported on a significant decrease in calpain10 gene expression in white blood cells (WBC) as well as the major insulin-target tissues including liver and adipose tissue, before the onset of diabetes in Otsuka Long-Evans Tokushima Fatty (OLETF) rats. In this study, we extended our hypothesis that some type 2 diabetes mellitus (NIDDM) susceptible genes are up/down-regulated before the onset in WBC of OLETF rats, reflecting their up/down-regulation in major insulin-target tissues, such as the liver. We tested this hypothesis using rat cDNA microarrays. The findings show that 1080 genes are up/down-regulated by more than 2-fold compared to the controls, Long-Evans Tokushima Otsuka rats, before the onset in WBC and liver under fasted or insulin administered condition. Fifty-seven of the 1080 genes were up/down-regulated in both WBC and the liver. More than half have been reported to NIDDM susceptible genes and the remainder have not been reported to be related to NIDDM. These results indicate that there some NIDDM related genes are up/down-regulated in WBC before the onset of diabetes.

Key words

white blood cell; non-insulin dependent diabetes mellitus; genetic diagnosis; cDNA microarray; Otsuka Long-Evans Tokushima fatty rat

	To cite this article:
	Shinya Iida, Yuichiro Sato, Akihiro Nakaya, Yasuo Shinohara, Yasuhiro Hayashi, Akihiro Sawada, Hideya Nagata, Noritada Kaji, Hiroyuki Kamiya, Yoshinobu Baba and Hideyoshi Harashima, “Genome Wide Expression Analysis of White Blood Cells and Liver of Pre-diabetic Otsuka Long-Evans Tokushima Fatty (OLETF) Rats Using a cDNA Microarray”, Biol. Pharm. Bull., Vol. 29, 2451-2459 (2006) .

	doi:10.1248/bpb.29.2451
	JOI JST.JSTAGE/bpb/29.2451