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ONLINEISSN:1348-0715
PRINTISSN:0366-7022
Chemistry Letters
Vol. 31 (2002) , No. 6 p.558
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Effect of Cyclen on the Prolongation of M Intermediate Lifetime in the D96N Mutant of Bacteriorhodopsin
Bing Liang1), Baofang Li1), Jianping Zhang1) and Long Jiang1)
1) Laboratory of Colloid and Interface, Center of Molecular Science, Institute of Chemistry the Chinese Academy of Sciences
(Received January 17, 2002)

A new kind of chemical additive—cyclen (1,4,7,10-tetra-azacyclododecane) was used in BRD96N-PVA films in an attempt to obtain longer M state lifetime. At the highest cyclen concentration of 1:250, the lifetime of M state could be prolonged to 936 s. Comparing the lifetimes of the M state in BRD96N suspension and BRD96N-PVA film with the same cyclen content, it is the strong complexing properties of cyclen towards H+ that plays the most important role in this prolongation.

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doi:10.1246/cl.2002.558
JOI  JST.JSTAGE/cl/2002.558
Copyright (c) 2002 The Chemical Society of Japan



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