CL : Vol. 32 (2003) , No. 2 p.176

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Chemistry Letters

Vol. 32 (2003) , No. 2 p.176

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Determination of Enzyme Immobilized into Electropolymerized Polymer Films

Saipin Thanachasai¹⁾, Hiroyasu Furukawa¹⁾, Shoichiro Yoshida¹⁾ and Tadashi Watanabe¹⁾

1) Institute of Industrial Science, The University of Tokyo

(Received November 7, 2002)

A simple, rapid and effective way was devised to determine an enzyme incorporated into electropolymerized conductive polymer films, by combining a thin-layer electropolymerization cell and the Bradford Coomassie colorimetric protein assay. The method has been successfully applied to the determination of horseradish peroxidase (HRP) entrapped into pyrrole and 3-alkylsulfonate pyrrole copolymer films.

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	doi:10.1246/cl.2003.176
	JOI JST.JSTAGE/cl/2003.176

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