ONLINE ISSN : 1347-4421 PRINT ISSN : 1389-1723

Journal of Bioscience and Bioengineering Vol. 91 (2001) , No. 1 103-105

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The Bacterium Burkholderia gladioli Strain CHB101 Produces Two Different Kinds of Chitinases Belonging to Families 18 and 19 of the Glycosyl Hydrolases MAKOTO SHIMOSAKA1), YASUHIRO FUKUMORI1), TAKUJI NARITA1), XIAO-YONG ZHANG1), RITSUKO KODAIRA1), MASAHIRO NOGAWA1) and MITSUO OKAZAKI2) 1) Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University 2) Gene Research Center, Shinshu University Two genes (chiA and chiB) coding for chitinases A and B (ChiA and ChiB) were isolated from the chitinolytic bacterium, Burkholderia gladioli strain CHB101. chiA contains an open reading frame that encodes a protein of 343 amino acids, whereas chiB encodes a protein of 307 amino acids. The deduced amino acid sequence of ChiA showed a high similarity to those of microbial chitinases belonging to family 18 of the glycosyl hydrolases, while ChiB showed significant sequence similarity to plant chitinases and Streptomyces spp. chitinases belonging to family 19. Key words:  Burkholderia gladioli, chitinase, chitosanase

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	To cite this article:
	MAKOTO SHIMOSAKA, YASUHIRO FUKUMORI, TAKUJI NARITA, XIAO-YONG ZHANG, RITSUKO KODAIRA, MASAHIRO NOGAWA and MITSUO OKAZAKI: “The Bacterium Burkholderia gladioli Strain CHB101 Produces Two Different Kinds of Chitinases Belonging to Families 18 and 19 of the Glycosyl Hydrolases”. J. BIOSCI. BIOENG., Vol. 91, 103-105 (2001) .

	doi:10.1263/jbb.91.103
	JOI  JST.JSTAGE/jbb/91.103

(c) 2001 The Journal of Bioscience and Bioengineering

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