抄録
In order to assess the body burden of 3, 5-di-tert-butyl-4-hydroxytoluene (BHT), human adipose tissues collected from 18 Japanese subjects during surgical treatment were analysed. The samples were extracted with hexane, the extracts were purified by Florisil column chromatography, and submitted to gas chromatographic determination via the derivation procedure. The recovery of BHT from human lipid was 66% at the level of 1 μg. BHT in the samples was confirmed by results of gas chromatography, thin-layer chromatography, and gas chromatography-mass spectrometry. BHT was found in all the samples analysed, with a maximum of 0.18 ppm and a mean of 0.10 ppm on fat basis.
