JSSSJ : Vol. 28 (2007) , No. 7 pp.385-390

SOI基板を用いたプレーナー型パッチクランプバイオセンサーの製作

浅野豪文¹⁾²⁾, 張振龍¹⁾, 宇野秀隆³⁾, 手老龍吾¹⁾³⁾, 鈴井光一³⁾, 中尾聡³⁾, 皆藤孝⁴⁾, 柴崎貢志¹⁾⁵⁾, 富永真琴¹⁾⁵⁾, 内海裕一⁶⁾, 宇理須恒雄¹⁾³⁾

1) 総合研究大学院大学物理科学研究科
2) 日本学術振興会特別研究員
3) 分子科学研究所
4) SIIナノテクノロジー(株)
5) 岡崎統合バイオサイエンスセンター
6) 兵庫県立大学高度産業科学技術研究所

(Received March 2, 2007)
(Accepted April 27, 2007)

Abstract
We have fabricated a planar type ion channel biosensor useful for the high throughput screening by using SOI (Silicon On Insulator) substrates. The micro-pore of about 1μm diameter was formed through the SOI substrate and controlled the structure of the pore. The substrate was assembled into the micro fluid circuit. Comparing with classical patch-clamp using the pipette, the planar type patch-clamp has advantages not only in the miniaturization and the high integration, but also in easiness of intracellular fluids exchange and of simultaneous measurement of ion channel current and fluoresce imaging. The HEK293 (Human Embryonic Kidney 293) cell with transfected TRPV1 (transient receptor potential vanilloid type 1) channels, which are mainly expressed in sensory nerve cells, was positioned on the micro-pore, and the capsaicin, which is a pungent ingredient of the hot pepper, was added to the external cell solution as a ligand molecule, then the whole-cell current of the ion channel was measured.

Keywords: planar patch-clamp, SOI substrate, biosensor, ion channel, TRPV1