1995 Volume 39 Issue 12 Pages 995-1001
The complete nucleotide sequence of the genomic RNA of odontoglossum ringspot virus Cy-1 strain (ORSV Cy-1) was determined using cloned cDNA. This sequence is 6611 nucleotides long containing four open reading frames, which correspond to 126K, 183K, 31K, and 18K proteins. Its genomic organization is similar to other tobamoviruses, TMV-V(vulgare), TMV-L (tomato strain), tobacco mild green mosaic virus (TMGMV) and cucumber green mottle mosaic virus (CGMMV). The 5' non-coding regions of ORSV Cy-1 is 62 nucleotides. The ORFs encoded a 126K polypeptide and a 183K read-through product in which helicase-sequence and polymerase-sequence motifs are found. The ORFs encoding the 126K and 183K proteins have 61% and 63% identities with those of TMV-V. The third ORF encoded a 31K protein homologous to TMV cell-to-cell movement protein. It has 63% identities with that of TMV-V. The fourth ORF encoded an 18K coat protein. The 5' non-coding region, which extends from base 1 to 62 has 2G residues and a ribosome binding site (AUU). The 3' non-coding region, 414 nucleotides in length, is entirely different from that of other tobamoviruses.
