1971 Volume 24 Issue 2 Pages 115-124
The dye and three different hemagglutination tests for toxoplasmosis were compared and evaluated in regard to the sensitivity and specificity on a total of 228 sera from humans, dogs and cats.
Good agreements were obtained among the positive rates by the dye test, a modified Jacobs-Lunde's and the Lewis-Kessel' s hemagglutination tests. Such a high agreement rate as 94% was shown on human sera by these hemagglutination tests, but slightly lower rates, 88.5% and 82.8%, on dog sera. The dye test demonstrated the antibody in all sera from proven cases of toxoplasmosis (29/29), whereas both Jacobs-Lunde's and Lewis-Kessel's hemagglutination tests in 97% (28/29) . Higher titers were demonstrated on the sera by these hemagglutination tests than by the dye test. By Jacobs-Lunde's hemagglutination test, the titer was 2 to 3 tubes (16 to 64 times) higher than that by the dye test. The titer by Lewis-Kessel's technique was 4 tubes (256 times) higher than that by the dye test. The Hanaki-Nobuto-Sato's hemagglutination test was slightly inferior to the other tests although the pre-sensitized and lyophilized erythrocytes are available for the test.
