Japanese Journal of Medical Science and Biology
Online ISSN : 1884-2828
Print ISSN : 0021-5112
ISSN-L : 0021-5112
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Volume 26, Issue 4
Displaying 1-3 of 3 articles from this issue
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  • R ANTIGEN OF VIBRIO CHOLERAE
    TOSHIO SHIMADA, RIICHI SAKAZAKI
    1973 Volume 26 Issue 4 Pages 155-160
    Published: 1973
    Released on J-STAGE: March 19, 2010
    DOIhttps://doi.org/10.7883/yoken1952.26.155
    JOURNAL FREE ACCESS
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    Serological properties of R antigen of Vibrio cholerae were studied with 46 strains representing each serotype of the species. Agglutination and agglutinin-absorption tests demonstrated that R antigens of all strains of V. cholerae were identical. It was also observed that most 0 antisera for V. cholerae contained some quantity of R antibody.
    No difference in colony morphology was recognized between the S parent and its R mutant cultures.
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  • CELLULAR AND HUMORAL IMMUNE RESPONSES IN MICE
    I. DEVELOPMENT OF DELAYED-TYPE FOOTPAD SWELLING AGAINST SHEEP ERYTHROCYTES AND ITS SUPPRESSION BY INTRAPERITONEAL ADMINISTRATION OF THE ANTIGEN
    SHIN-ICHI TAMURA, TAKESHI KURATA, MASANOBU SUGIMOTO, YASUYUKI EGASHIRA
    1973 Volume 26 Issue 4 Pages 161-168
    Published: 1973
    Released on J-STAGE: March 19, 2010
    DOIhttps://doi.org/10.7883/yoken1952.26.161
    JOURNAL FREE ACCESS
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    A delayed hypersensitivity reaction to sheep red blood cells (SRBC) was elicited in the footpads of the mice sensitized by subcutaneous (sc) injection of the antigen emulsified in Freund's complete adjuvant (FCA) ; injection of the test antigen to the footpad induced a strong reaction with its peak at 24 hr. The delayed hypersensitivity was first detected on day 3 and reached its maximum on day 5 after sensitization, accompanying no serum antibody production. On the other hand, a humoral response with no delayed hypersensitivity was induced by intraperitoneal (ip) injection of SRBC without FCA.
    The delayed hypersensivity was suppressed by ip injection of SRBC given during the period of 7 days before to 2 days after the sensitization by sc injection of the antigen in FCA. However, the humoral response induced by ip injection of SRBC was not affected by sc injection of SRBC in FCA. It was suggested that even if the appearance of the delayed hypersensitivity reaction was suppressed by ip injection of the antigen, sc injection of SRBC in FCA was capable of bestowing the immunological memory for delayed hypersensitivity on the mouse.
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  • LONG-TERM CULTIVATION OF FIBROBLASTS DERIVED FROM NORMAL GINGIVAL TISSUES OF HUMAN ADULTS
    TAKEKI TSUTSUI, YASUKO HIROKAWA, HEIJI MAIZUMI
    1973 Volume 26 Issue 4 Pages 169-177
    Published: 1973
    Released on J-STAGE: March 19, 2010
    DOIhttps://doi.org/10.7883/yoken1952.26.169
    JOURNAL FREE ACCESS
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    Human fibroblasts derived from normal gingival tissues of 12 adults were continuously cultivated in three different synthetic media (199, F12 and RPMI 1640 medium) supplemented with 20% calf serum or 10% fetal bovine serum and 5μg/ml fetuin. Investigations on the cumulative growth with eight strains found that RPMI 1640 medium was most suitable for long-term cultivation. Almost all of 17 strains grew for over one year under these conditions.
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