Rapid Separation of Triacylglycerol Positional Isomers Binding Two Saturated Fatty Acids Using Octacocyl Silylation Column

Toshiharu Nagai; Naohiro Gotoh; Hoyo Mizobe; Kazuaki Yoshinaga; Koichi Kojima; Yumiko Matsumoto; Shun Wada

doi:10.5650/jos.60.345

Oils and Fats

Rapid Separation of Triacylglycerol Positional Isomers Binding Two Saturated Fatty Acids Using Octacocyl Silylation Column

Toshiharu Nagai, Naohiro Gotoh, Hoyo Mizobe, Kazuaki Yoshinaga, Koichi Kojima, Yumiko Matsumoto, Shun Wada

著者情報

キーワード: octacocyl silylation column, saturated fatty acid, separation, triacylglycerol positional isomer, unsaturated fatty acid

ジャーナルフリー

2011 年 60 巻 7 号 p. 345-350

DOI https://doi.org/10.5650/jos.60.345

詳細

抄録

The rapid separation of a triacylglycerol positional isomer (TAG-PI) pair was examined via high-performance liquid chromatography–atmospheric pressure chemical ionization tandem mass spectrometry using an octacocyl silylation (C28) column. A TAG-PI pair binding two palmitic acids and one fatty acid that structurally differs from palmitic acid was separated at 10°C and 15°C using acetone as the mobile phase. However, the TAG-PI pair binding two unsaturated fatty acids and one saturated fatty acid was not separated by the C28 column. The results indicate that the structures of the two palmitic acids (saturated fatty acids) and the other fatty acid at the α or β position in TAG play an important role in the separation of the TAG-PI pair, and that the structure of the fatty acid needs to be considerably different from that of the palmitic acid, specifically in terms of the chain length or the location of the double bond.

責任著者(Corresponding author)

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