Apoptosis and surfactant protein-C expression inhibition induced by lipopolysaccharide in AEC II cell may associate with NF-κB pathway

Jinle Lin; Jun Tian; Li Wang; Weigang Wu; Huaying Li; Xueyan Wang; Xiaobin Zeng; Wenwu Zhang

doi:10.2131/jts.42.53

Original Article

Apoptosis and surfactant protein-C expression inhibition induced by lipopolysaccharide in AEC II cell may associate with NF-κB pathway

Jinle Lin, Jun Tian, Li Wang, Weigang Wu, Huaying Li, Xueyan Wang, Xiaobin Zeng, Wenwu Zhang

著者情報

Jinle Lin
Department of Emergency and Critical Care Medicine, Baoan Hospital, Nanfang Medical University, China
Jun Tian
Key Lab for New Drug Research of TCM and Shenzhen Branch, State R&D Centre for Viro-Biotech, Research Institute of Tsinghua University in Shenzhen, China
College of Life Science, Jiangsu Normal University, China
Li Wang
Department of Emergency and Critical Care Medicine, Baoan Hospital, Nanfang Medical University, China
Weigang Wu
Central Lab of Longhua Branch, Shenzhen People’s Hospital, 2nd Clinical Medical College of Jinan University, China
Huaying Li
Central Lab of Longhua Branch, Shenzhen People’s Hospital, 2nd Clinical Medical College of Jinan University, China
Xueyan Wang
Key Lab for New Drug Research of TCM and Shenzhen Branch, State R&D Centre for Viro-Biotech, Research Institute of Tsinghua University in Shenzhen, China
Xiaobin Zeng
Central Lab of Longhua Branch, Shenzhen People’s Hospital, 2nd Clinical Medical College of Jinan University, China
Key Lab for New Drug Research of TCM and Shenzhen Branch, State R&D Centre for Viro-Biotech, Research Institute of Tsinghua University in Shenzhen, China
Wenwu Zhang
Department of Emergency and Critical Care Medicine, Baoan Hospital, Nanfang Medical University, China

キーワード: Acute respiratory distress syndrome (ARDS), Lipopolysaccharide, NF-κB pathway, Apoptosis, Surfactant Protein-C

ジャーナルフリー

2017 年 42 巻 1 号 p. 53-61

DOI https://doi.org/10.2131/jts.42.53

詳細

抄録

Lipopolysaccharide (LPS), a Gram-negative bacterial outer membrane component, is one of the major causes of septic shock. Herein we investigate LPS-induced apoptosis of rat alveolar epithelial type II cells (AEC II) and the effects of LPS on surfactant protein-C (SP-C) expression in AEC II, along with the possible molecular mechanisms. LPS exposure impaired cell viability and increased apoptosis of AEC II significantly in concentration-dependent manner embodied in increased caspase-3 expression and the activity of caspase-3. Simultaneously, our results also indicated that LPS inhibited surfactant protein-C (SP-C) expression in AEC II. Mechanistic studies revealed that LPS treatment significantly increased the expression of NF-κB p50, NF-κB p65 and IKKβ proteins as well as induced IκB-α phosphorylation. Moreover, pretreatment with IKK inhibitor IKK-16 or NF-κB inhibitor PDTC ameliorated LPS-caused alterations in cleaved caspase-3 expression, the activity of caspase-3 and SP-C expression. Taken together, these results demonstrate that LPS can induce apoptosis of AEC II and decrease SP-C expression partly through activating the NF-κB pathway.

責任著者(Corresponding author)

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