As a cellulose acetate membrane for serum protein fractionation testing, SELECA-VSP has been developed to substitute for SEPARAX-SP. In order to investigate whether or not SELECA-VSP is available for routine laboratory testing, we evaluated basic performance of SELECA-VSP by use of automated electrophoresis system, Olympus AES series.
As for the consecutive transportability, no jam was occurred on 300 consecutive membrane sheets of SELECA-VSP, lot T13-2. Reproducibility was good in within-run and between-run precision. However, the waiting coefficient for application should be set up adequately in each laboratory because SELECA-VSP tends not to dry up. Using SELECA-VSP the decolored clear zone between β globulin fraction and γ globulin fraction is more distinct than SEPARAX-SP, the results analyzed by diagnosis support program may be misinterpreted. In order to avoid the misinterpretation, the standard densitogram of normal control by use of SELECA-VSP should be prepared.