生物物理化学
Online ISSN : 1349-9785
Print ISSN : 0031-9082
ISSN-L : 0031-9082
26 巻, 5 号
選択された号の論文の11件中1~11を表示しています
  • 小林 貞男
    1982 年 26 巻 5 号 p. 333-334
    発行日: 1982/10/25
    公開日: 2009/03/31
    ジャーナル フリー
  • 島尾 和男
    1982 年 26 巻 5 号 p. 335-339
    発行日: 1982/10/25
    公開日: 2009/03/31
    ジャーナル フリー
  • 馬場 巽, 馬場 三和, 唐下 博子, 一村 光子
    1982 年 26 巻 5 号 p. 341-346
    発行日: 1982/10/25
    公開日: 2009/03/31
    ジャーナル フリー
  • 福田 守道, 山田 正二
    1982 年 26 巻 5 号 p. 347-355
    発行日: 1982/10/25
    公開日: 2009/03/31
    ジャーナル フリー
  • 佐野 紀代子, 長 裕子, 芝 〓彦, 中尾 真
    1982 年 26 巻 5 号 p. 357-362
    発行日: 1982/10/25
    公開日: 2009/03/31
    ジャーナル フリー
  • 門福 強樹, 真鍋 敬, 佐藤 永雄, 奥山 典生
    1982 年 26 巻 5 号 p. 363-369
    発行日: 1982/10/25
    公開日: 2009/03/31
    ジャーナル フリー
    We used fluorescein isothiocyanate (FITC) as a stain for the detection of two-dimensionally separated proteins. Human serum proteins were pre-labeled with FITC by mixing serum and a FITC solution at 0°C and pH 9.2 for 24 hours and subjected to two-dimensional electrophoresis in the absence of denaturing agents. The distribution of labeled proteins on the slab gel could be observed during and just after the electrophoretic run by irradiation with UV light.
    When the FITC concentration was below 3.5mM in serum-FITC mixture, the mobilities of serum proteins did not differ from those of the native proteins. By controlling the FITC concentration, selective labeling of specific serum proteins was possible
  • 海上 智, 平間 とも子, 谷本 義文
    1982 年 26 巻 5 号 p. 371-377
    発行日: 1982/10/25
    公開日: 2009/03/31
    ジャーナル フリー
    The biochemical properties of creatine kinase (CK) isoenzymes were compared in various animals and the following characteristics were noted. 1. Isoenzyme electrophoretic patterns with CK1 and CK3 were observed in rat, rabbit, dog, miniature pig, mouse, and monkey plasma. 2. The activity of platelet rich plasma CK was much higher than that of the corresponding platelet poor plasma enzyme in rabbit, dog, rat, and miniature pig. 3. Plasma CK was markedly different in substrate affinity among the species. The apparent Km values well correlated with the percentage of CK1 or CK3 fraction in the plasma of various animals. 4. Upon incubation at 37°C, CK activities in rat and mouse plasma decreased rapidly, and the recovery of enzyme activities was only slight after the addition of sulfhydryl agents. On the other hand, in the presence of sulfhydryl agents, CK in human and monkey plasma were completely protected from loss of activity at 37°C.
  • 井上 雅公, 荘野 哲朗, 古賀 俊逸, 井林 博
    1982 年 26 巻 5 号 p. 379-383
    発行日: 1982/10/25
    公開日: 2009/03/31
    ジャーナル フリー
    We established a simple method for measuring HDL subfraction cholesterols by gradient polyacrylamide gel electrophoresis (gradient PAGE). Prestained human serum was separated into two major bands identical with HDL2 and HDL3 by the gradient PAGE. HDL-cholesterol (HDL-C) obtained by precipitation method was divided according to HDL2/HDL3 ratio determined by densitometry of the electrophoretic pattern. HDL subfraction cholesterols determined by this method was well correlated with those measured by zonal ultracentrifugation (HDL2-C: r=0.98, HDL3-C: r=0.94, n=11). Mean serum HDL2-C level was significantly higher in normal females than in normal males (p<0.01). HDL3-C levels were significantly decreased in liver diseases, especially in cirrhotics. This simple electrophoretic method for quantification of HDL2 and HDL3 is suitable for clinical application in various pathological states.
  • 門脇 武博, 吉田 光孝, 稲福 全昌, 高田 勗
    1982 年 26 巻 5 号 p. 385-391
    発行日: 1982/10/25
    公開日: 2009/03/31
    ジャーナル フリー
    マウスが胎児期から発育するに伴って,生体内の多分子型酵素の変動がどのように起っているのかを知るために,ddn系マウスを用いて各種組織LDHアイソザイムについて調べた.マウスのLDHはすでにMarkertらによって報告されているが,われわれは新たに大脳,小脳,肺臓を追加し,胎児期から成熟期に至る7発育段階における詳細な実験を行った.
    胎児期の妊娠19日目の各組織のLDH活性値は肝臓>心臓>骨格筋>腎臓>大脳>肺臓>小脳の順であり,成熟期56日目では腎臓>骨格筋>心臓>肝臓>大脳>小脳>肺臓の順であった.また,妊娠19日目と生後7日目の各組織のLDH活性値を比較すると,腎臓,肝臓,骨格筋の組織では増加するのに対し,大脳,小脳,心臓,肝臓の組織では減少がみられた.
    妊娠12日目の各組織のLDHアイソザイムの主分画はすべてLDH5であったが,生後7日目では大脳はLDH4に,小脳と心臓はLDH3に,腎臓はLDH2に主分画が変動を示した.成熟期では大脳は生後56日目で主分画はLDH3,小脳は生後21日目でLDH1,心臓および腎臓は生後21日目でLDH2を示した.また,肺臓,骨格筋,肝臓では胎児期12日目から成熟期においてもLDH5が主分画であった.
    これらLDHアイソザイムの変動はマウスの発育環境に適応して変動しているのではないかと推定される.
  • 松尾 好祥, 鈴木 盛一, 榊原 泉, 雨宮 浩
    1982 年 26 巻 5 号 p. 393-396
    発行日: 1982/10/25
    公開日: 2009/03/31
    ジャーナル フリー
    α1-Antitrypsin (α1-AT) was isolated from normal human plasma by the method described by Pannel et al. Effect of isolated α1-AT treatment of lymphocytes on the immune response of normal human peripheral blood lymphocytes was studies by plaque-forming cells assay using protein-A coated neuraminidase treated goat erythrocyte. α1-AT was found to have suppressive effect on immunoglobulin production of normal human peripheral blood lymphocyte stimulated with pokeweed mitogen. The suppressive effect of α1-AT treated B cell was stronger than that of α1-AT treated T cell.
  • 佐野 紀代子, 長 裕子, 芝 〓彦, 中尾 真
    1982 年 26 巻 5 号 p. 397-402
    発行日: 1982/10/25
    公開日: 2009/03/31
    ジャーナル フリー
    Analysis of serum γ-GTP isoenzymes was attempted with isoelectric focusing using agarose gel as a supporting medium. After testing various conditions to obtain electrophoretic patterns with good reproducibility, suspension of Ampholine in Agarose IEF was finally chosen as a supporting medium, and electrolysis at 6.25W/plate (length 12.5cm×width 11cm) for 1hr under cooling with circulating water was found to be most appropriate. A serum sample, 20μl, absorbed to a small piece of filter paper was applied onto the medium 1cm away from the cathode. Under these conditions, the pH gradient was linear in the range from pH 3.5 to 9.5, and serum proteins were clearly separated.
    This method was applied to the separation of γ-GTP isoenzymes. γ-GTP was stained with γ-L-glutamyl-p-diethylaminoanilide. After electrophoresis, the gel was fixed with 50% saturated ammonium sulfate solution followed by removal of the ammonium sulfate, and then submitted to staining procedures. This pretreatment was found to give very clear and permanently preservable electrophoretic patterns. Serum γ-GTP was separeted into 7 isoenzyme bands by this method. The bands which consistently appeared were in pI range of 4.5-5.0, while a band at pI 5.5 was noted in the cases of bile stagnation, and a band at pI 6.5 was observed in cases with malignant tumors.
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