A new procedure for confirmation of focusing pattern after preparative agarose gel isoelectric focusing is described. The preparative gel was covered with a thin layer agarose gel (cover gel) from the start of focusing. A printing procedure can be omitted in this procedure since the cover gel is stained after focusing. The focused cover gel pattern is more clear than the printed cellulose acetate membrane. When the dry cover gel was used, the transcription volume was a little and the recovery of protein from the preparative gel was similar to the printing procedure with the membrane. In spite of removing carbonic acid gas with the utmost care, skewed protein bands were sometimes observed and its direction of skew was irregular. Therefore, it was assumed that there are some factors caused skewed protein bands besides carbonic acid gas.