Capillary type isotachophoresis (CITP) and micro two-dimentional polyacrylamide gel electrophoresis (M2D-PAGE) were used by examining the genetic polymorphisms and the physiological variation of horse serum proteins. Both methods were useful for evaluating Tf typings. Especially, the CITP was a rapid analytical method. One of the very rare Tf type, Tf DM, which was undetectable by conventinal methods, was discovered by using this method. The amount of various proteins were estimated from the data on UV absorption. The Tf heterogenity was detectable by the M2D-PAGE. In addition, this method proved to be an excellent one for observing physiological conditions of each samples.