During fifty years' history of the Japanese Electrophoresis Society, many types of handmade electrophoresis experiments by many members of the Society contributed to the progress of electrophoretic methods. Starting from the handmade Tiselius' electrophoresis apparatus by Hirai and Shimao, recollections of the members active in the handmade electrophoreses in the first fifteen years of the Society are described. Then, two examples of handmade electrophoresis by the author, namely, a buffer gradient gel electrophoresis and a comparative study of computer simulations of glycine and tricine discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis made in the last twenty years are shown.