Applied Entomology and Zoology
Online ISSN : 1347-605X
Print ISSN : 0003-6862
ISSN-L : 0003-6862
Regular Papers
Formulation of highly volatile pheromone of the white grub beetle Dasylepida ishigakiensis (Coleoptera: Scarabaeidae) to develop monitoring traps
Sadao Wakamura, Hiroe Yasui, Fumiaki Mochizuki, Takehiko Fukumoto, Norio Arakaki, Atsushi Nagayama, Takumi Uesato, Akiko Miyagi, Hiroaki Oroku, Seiji Tanaka, Makoto Tokuda, Midori Fukaya, Toshiharu Akino, Yoshio Hirai, Masakazu Shiga
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Keywords: Dasylepida ishigakiensis, sex attractant pheromone, polyethylene tube formulation, (R)-2-butanol, highly volatile
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2009 Volume 44 Issue 4 Pages 579-586

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Abstract

Polyethylene tube formulation was developed for the highly volatile sex pheromone of the white grub beetle Dasylepida ishigakiensis, a serious sugarcane pest that appears above the ground for mating during a short period on Miyako Island, Okinawa, Japan. A polyethylene tube 1 cm long containing 12 mg (R)-2-butanol was found to be one of the most effective formulations for attracting and capturing male beetles when baited in a funnel trap in the field. Its attractiveness was comparable to that of a caged virgin female. Similarly prepared tubes containing (S)-2-butanol and 2-propanol showed no attractiveness. As for the enantiomeric purity of (R)-2-butanol, blending 10% or less (S)-2-butanol did not affect attractiveness, whereas no male beetles were captured with the racemic mixture. Blending (R)-2-butanol with 2-propanol in a ratio of 1 : 2 or less showed neither a synergistic nor inhibitory effect on beetle catches in the field, while the release rate of 2-propanol from the tube was approximately 50–60% of that of 2-butanol. A tube lure containing 12 mg (R)-2-butanol released ca. 50 ng of this compound per min at 20°C in the laboratory. This led to the estimation that the effective duration of this lure (>5 months) would be sufficient to cover the entire flight season of D. ishigakiensis beetles.

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© 2009 by the Japanese Society of Applied Entomology and Zoology
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