The paper wasp Polistes chinensis antennalis is characterized by a high incidence of worker oviposition, even in the presence of their original queen. To analyze the fine-scale genetic structure within colonies, we isolated nine novel microsatellite loci for this species, for which 13 microsatellite loci were reported. No linkage disequilibrium was detected among all 22 loci. The primer pairs described here are useful in analyzing the colony and population genetic structures of this species.