Enhancement of the Binding Ability of a Ligand for Nucleobase Recognition by Introducing a Methyl Group

Qing DAI; Chun-Yan XU; Yusuke SATO; Keitaro YOSHIMOTO; Seiichi NISHIZAWA; Norio TERAMAE

doi:10.2116/analsci.22.201

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Enhancement of the Binding Ability of a Ligand for Nucleobase Recognition by Introducing a Methyl Group

Qing DAI, Chun-Yan XU, Yusuke SATO, Keitaro YOSHIMOTO, Seiichi NISHIZAWA, Norio TERAMAE

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JOURNAL FREE ACCESS

2006 Volume 22 Issue 2 Pages 201-203

DOI https://doi.org/10.2116/analsci.22.201

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Abstract

The recognition ability of pteridine derivatives for nucleobases opposite an abasic (AP) site in an oligodeoxynucleotide (ODN) duplex is enhanced by using a propylene residue (Spacer-C3) as an AP site. The recognition ability is further enhanced both by attaching methyl groups to a fluorescent ligand and by measuring the fluorescence response at 5°C; 6.2 × 10⁶ M^-1 of the binding constant is attained between 2-amino-6,7-dimethyl-4-hydroxypteridine and guanine opposite the AP site in water.

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