Analytical Sciences
Online ISSN : 1348-2246
Print ISSN : 0910-6340
ISSN-L : 0910-6340
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Comparative Studies on Effects of Acid Solutions on Aquatic Plants by Beam Deflection and Absorbance Spectroscopy Methods
Xing-Zheng WULiangjiao NIETomomi INOUE
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2015 Volume 31 Issue 8 Pages 837-840

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Abstract

The beam deflection method and absorbance spectroscopy were applied to study effects of acid solutions on aquatic plants, and their results were compared. Aquatic plants Egeria densa and Ceratophyllum demersum L were used as model plants. In absorbance experiments, a piece of the plants was put in a beaker with 20 mL HCl solution, and absorbance of the HCl solution was measured every 30 min. In beam deflection experiments, a probe beam from a He-Ne laser was focused to a vicinity of the plants in a culture dish with HCl solution by an objective lens, and deflection signals of the probe beam were monitored by a position sensor. Absorbance spectra of the HCl solutions with immersing of the plants showed absorbance below 410 nm, suggesting that some compounds leaked from the plants into the HCl solutions. Changes of absorbance and deflection signals with immersion time were examined for different pH levels. The changing trends of the absorbance and deflection signals with time were similar, but the absorbance changes were delayed for about 2 – 3 h. The absorbance method could not detect the effect of the pH 5.0 HCl solutions on the aquatic plants, while the deflection method could.

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© 2015 by The Japan Society for Analytical Chemistry
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