Evaluation of a Portable Microchip Electrophoresis Fluorescence Detection System for the Analysis of Amino Acid Neurotransmitters in Brain Dialysis Samples

Nathan J. OBORNY; Elton E. Melo COSTA; Leena SUNTORNSUK; Fabiane C. ABREU; Susan M. LUNTE

doi:10.2116/analsci.32.35

Original Papers

Evaluation of a Portable Microchip Electrophoresis Fluorescence Detection System for the Analysis of Amino Acid Neurotransmitters in Brain Dialysis Samples

Nathan J. OBORNY, Elton E. Melo COSTA, Leena SUNTORNSUK, Fabiane C. ABREU, Susan M. LUNTE

Author information

Keywords: Microchip electrophoresis, amino acid neurotransmitter, microdialysis, fluorescence detection, naphthalene-2,3-dicarboxaldehyde, neuroactive amine

JOURNAL FREE ACCESS

2016 Volume 32 Issue 1 Pages 35-40

DOI https://doi.org/10.2116/analsci.32.35

Details

Abstract

A portable fluorescence detection system for use with microchip electrophoresis was developed and compared to a benchtop system. Using this system, six neuroactive amines commonly found in brain dialysate (arginine, citrulline, taurine, histamine, glutamate, and aspartate) were derivatized offline with naphthalene-2,3-dicarboxaldehyde/cyanide, separated electrophoretically, and detected by fluorescence. The limits of detection for the analytes of interest were 50 – 250 nM for the benchtop system and 250 nM – 1.3 μM for the portable system, both of which were adequate for most analyte detection in brain microdialysis samples. The portable system was then demonstrated for the detection of the same six amines in a rat brain microdialysis sample.

Fullsize Image

Corresponding author

Register with J-STAGE for free!