Quantitative Live Single-cell Mass Spectrometry with Spatial Evaluation by Three-Dimensional Holographic and Tomographic Laser Microscopy

Ahmed ALI; Yasmine ABOULEILA; Sara AMER; Rie FURUSHIMA; Samy EMARA; Sebastien EQUIS; Yann COTTE; Tsutomu MASUJIMA

doi:10.2116/analsci.32.125

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Quantitative Live Single-cell Mass Spectrometry with Spatial Evaluation by Three-Dimensional Holographic and Tomographic Laser Microscopy

Ahmed ALI, Yasmine ABOULEILA, Sara AMER, Rie FURUSHIMA, Samy EMARA, Sebastien EQUIS, Yann COTTE, Tsutomu MASUJIMA

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Keywords: Quantitative live single-cell mass spectrometry, three-dimensional imaging, holographic and tomographic microscopy, quantitative single-cell analysis, HepG2 cell

JOURNAL FREE ACCESS

2016 Volume 32 Issue 2 Pages 125-127

DOI https://doi.org/10.2116/analsci.32.125

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Abstract

The locations and volumes of the contents of a single HepG2 cell were visualized under three-dimensional (3D) holographic and tomographic (HT) laser microscopy, colored by refractive index, not staining. After trapping the specific area of a target cell in a nanospray tip, quantification was performed by live single-cell mass spectrometry. Comparison of the HepG2 cells’ before and after 3D-HT images allowed the inference of the precise volume and original location of the trapped cell contents. The total amount of a trapped molecule was estimated. The images also revealed morphological changes in the cell structure caused by the manipulation.

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