Brasilicardin A, a New Terpenoid Antibiotic from Pathogenic Nocardia brasiliensis: Fermentation, Isolation and Biological Activity

HISAYUKI KOMAKI; AKIRA NEMOTO; YASUSHI TANAKA; HIROAKI TAKAGI; KATSUKIYO YAZAWA; YUZURU MIKAMI; HIDEYUKI SHIGEMORI; JUN'ICHI KOBAYASHI; AKIKAZU ANDO; YOSHIHO NAGATA

doi:10.7164/antibiotics.52.13

Brasilicardin A, a New Terpenoid Antibiotic from Pathogenic Nocardia brasiliensis: Fermentation, Isolation and Biological Activity

HISAYUKI KOMAKI, AKIRA NEMOTO, YASUSHI TANAKA, HIROAKI TAKAGI, KATSUKIYO YAZAWA, YUZURU MIKAMI, HIDEYUKI SHIGEMORI, JUN'ICHI KOBAYASHI, AKIKAZU ANDO, YOSHIHO NAGATA

Author information

HISAYUKI KOMAKI
Research Laboratory, Higeta Shoyu Co., Ltd.
Department of Biotechnology, Graduate School of Science and Technology, Chiba University
AKIRA NEMOTO
Research Laboratory, Higeta Shoyu Co., Ltd.
YASUSHI TANAKA
Research Laboratory, Higeta Shoyu Co., Ltd.
HIROAKI TAKAGI
Research Laboratory, Higeta Shoyu Co., Ltd.
KATSUKIYO YAZAWA
Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University
YUZURU MIKAMI
Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University
HIDEYUKI SHIGEMORI
Graduate School of Pharmaceutical Sciences, Hokkaido University
JUN'ICHI KOBAYASHI
Graduate School of Pharmaceutical Sciences, Hokkaido University
AKIKAZU ANDO
Department of Biotechnology, Graduate School of Science and Technology, Chiba University
YOSHIHO NAGATA
Department of Biotechnology, Graduate School of Science and Technology, Chiba University

JOURNAL FREE ACCESS

1999 Volume 52 Issue 1 Pages 13-19

DOI https://doi.org/10.7164/antibiotics.52.13

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Abstract

A novel tricyclic diterpenoid antibiotic, brasilicardin A, was isolated from the culture broth of Nocardia brasiliensis IFM 0406. The antibiotic exhibited immunosuppressive activity in a mouse mixed lymphocyte reaction (MLR) assay system and its IC₅₀ value was 0.057 μg/ml. Although the inhibitory activity of cyclosporin A (CyA) against IL-2 production was confirmed in the MLR assay system, brasilicardin A did not have the activity. The results of in vitro toxicity testing of brasilicardin A against various human cell lines were compared with those of CyA.

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