The Journal of Antibiotics
Online ISSN : 1881-1469
Print ISSN : 0021-8820
ISSN-L : 0021-8820
Cloning and Expression of a Gene Encoding N-Glycosyltrasferase (ngt) from Saccharothrix aerocolonigenes ATCC39243
TAKESHI OHUCHIATSUKO IKEDA-ARAKIAYAKO WATANABE-SAKAMOTOKATSUHISA KOJIRIMASAO NAGASHIMAMASANORI OKANISHIHIROYUKI SUDA
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2000 Volume 53 Issue 4 Pages 393-403

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Abstract

In the course of our bioconversion studies on the derivatives of an indolocarbazole, J-104303, Saccharothrix aerocolonigenes ATCC39243 was found to convert J-104303, which was added into the culture medium, to its glycosylated derivative, J-1093 84. In order to clone the gene having the ability to convert J-104303 to J-109384, a library of Saccharothrix aerocolonigenes ATCC39243 DNA fragments was constructed using Streptomyces lividans TK21 and pIJ702 as host strain and vector, respectively. By examining more than 5, 000 transformants, one was found to convert J-104303 to J-109384. Sequence analysis of the inserted DNA fragment revealed an open reading frame with 1, 245 base pairs, named ngt. The transformant containing this ngt gene was also found to introduce a D-glucose moiety into 6-N-methylarcyriaflavin C. Furthermore, when ngt was introduced into Streptomyces mobaraensis BA13793, a producer of J-104303, the resulting transformant produced J-109384 directly.

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