Substrate Inhibition of <small>L</small>-Cysteine α,β-Elimination Reaction Catalyzed by <small>L</small>-Cystathionine γ-Lyase of Saccharomyces cerevisiae

Shuzo YAMAGATA; Motomi ISAJI; Tomoko YAMANE; Tomonori IWAMA

doi:10.1271/bbb.66.2706

Biochemistry & Molecular Biology Notes

Substrate Inhibition of L-Cysteine α,β-Elimination Reaction Catalyzed by L-Cystathionine γ-Lyase of Saccharomyces cerevisiae

Shuzo YAMAGATA, Motomi ISAJI, Tomoko YAMANE, Tomonori IWAMA

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Keywords: cysteine β-elimination, cystine β-elimination, cystathionine γ-lyase, pyridoxal 5′-phosphate, Saccharomyces cerevisiae

JOURNAL FREE ACCESS

2002 Volume 66 Issue 12 Pages 2706-2709

DOI https://doi.org/10.1271/bbb.66.2706

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Abstract

The α,β-elimination of L-cysteine catalyzed by Saccharomyces cerevisiae L-cystathionine γ-lyase (EC 4.4.1.1) was inhibited by the substrate. The absorption spectrum of the holoenzyme in the presence of L-cysteine showed that the substrate inhibition observed in this reaction was due mainly to removal of the cofactor.

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