Preparation of Protein Transduction Domain-Fused Peptidyl Prolyl cis/trans Isomerase Pin1

Tsubasa OHASHI; Sumito TESHIMA; Masafumi HIDAKA; Takafumi UCHIDA

doi:10.1271/bbb.100372

Biochemistry & Molecular Biology Regular Papers

Preparation of Protein Transduction Domain-Fused Peptidyl Prolyl cis/trans Isomerase Pin1

Tsubasa OHASHI, Sumito TESHIMA, Masafumi HIDAKA, Takafumi UCHIDA

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Keywords: protein transduction domain human immunodeficiency virus-1 trans-actuvatir if transcription factor (TAT), peptidyl prolyl cis/trans isomerase Pin1, cell cycle

JOURNAL FREE ACCESS

2010 Volume 74 Issue 10 Pages 2067-2070

DOI https://doi.org/10.1271/bbb.100372

View "Advance Publication" version (October 7, 2010).

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Abstract

The phenotypes of mice lacking peptidyl prolyl cis/trans isomerase Pin1 (Pin1^−⁄−) indicated that deficient Pin1 might be related to a variety of diseases. We created TAT-Pin1, a fusion protein of human immunodeficiency virus 1 trans-activator of transcription factor with Pin1. Treatment of HeLa cells with TAT-Pin1 increased the ratio of the S phase. Moreover, TAT-Pin1 restored the proliferating function of Pin1^−⁄− mouse embryonic fibroblasts which cannot restart proliferation after G0 arrest. These results indicate that TAT-Pin1 is useful in studying the functions of Pin1 and can be developed as a macromolecular drug for diseases related to Pin1 loss.

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