Leaf Extract of Wasabia japonica Relieved Oxidative Stress Induced by Helicobacter pylori Infection and Stress Loading in Mongolian Gerbils

Hirotaka SEKIGUCHI; Fumiyo TAKABAYASHI; Yuya DEGUCHI; Hideki MASUDA; Tomoyasu TOYOIZUMI; Shuichi MASUDA; Naohide KINAE

doi:10.1271/bbb.90919

Food & Nutrition Science Regular Papers

Leaf Extract of Wasabia japonica Relieved Oxidative Stress Induced by Helicobacter pylori Infection and Stress Loading in Mongolian Gerbils

Hirotaka SEKIGUCHI, Fumiyo TAKABAYASHI, Yuya DEGUCHI, Hideki MASUDA, Tomoyasu TOYOIZUMI, Shuichi MASUDA, Naohide KINAE

Author information

Hirotaka SEKIGUCHI
Department of Food and Nutritional Sciences, Graduate School of Nutritional and Environmental Sciences, and Global COE program, University of Shizuoka
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University
Fumiyo TAKABAYASHI
University of Shizuoka, Junior College
Yuya DEGUCHI
Department of Food and Nutritional Sciences, Graduate School of Nutritional and Environmental Sciences, and Global COE program, University of Shizuoka
Faculty of Pharmaceutical Sciences, Nagasaki International University
Hideki MASUDA
Department of Food and Nutritional Sciences, Graduate School of Nutritional and Environmental Sciences, and Global COE program, University of Shizuoka
Tomoyasu TOYOIZUMI
Department of Food and Nutritional Sciences, Graduate School of Nutritional and Environmental Sciences, and Global COE program, University of Shizuoka
Laboratory of Genetics, Division of Toxicology, Hatano Research Institute, Food and Drug Safety Center
Shuichi MASUDA
Department of Food and Nutritional Sciences, Graduate School of Nutritional and Environmental Sciences, and Global COE program, University of Shizuoka
Naohide KINAE
Department of Food and Nutritional Sciences, Graduate School of Nutritional and Environmental Sciences, and Global COE program, University of Shizuoka

Keywords: Helicobacter pylori, Mongolian gerbils, 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG), oxidative DNA damage, Wasabia japonica

JOURNAL FREE ACCESS

2010 Volume 74 Issue 6 Pages 1194-1199

DOI https://doi.org/10.1271/bbb.90919

View "Advance Publication" version (June 7, 2010).

Details

Abstract

Infection with Helicobacter pylori (H. pylori) can induce gastric disorders, and though its presence cannot explain disease pathogenesis and does not have associations with other factors, it is well known that H. pylori infection causes stomach inflammation following oxidative stress. We examined the suppressive effects of a leaf extract of Wasabia japonica on H. pylori infection and on stress loading in Mongolian gerbils. Following oral administration of wasabi extract of 50 and 200 mg/kg B.W./d for 10 d, the animals were exposed to restraint stress for 90 and 270 min. As for the results, the level of 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) in the stomach and oxidative DNA damage in peripheral erythrocytes at 270 min significantly increased. That elevation was significantly suppressed by the addition of the leaf extract. We concluded that the simultaneous loading of H. pylori infection and physical stress loading might induce oxidative DNA damage additively, while a leaf extract attenuated this DNA damage in the stomach as well as the peripheral erythrocytes.

Corresponding author

Register with J-STAGE for free!