Radical Scavenging Activity of Spring Mountain Herbs in the Shikoku Mountain Area and Identification of Antiradical Constituents by Simple HPLC Detection and LC-MS Methods

Toshiya MASUDA; Tomoko INOUCHI; Aya FUJIMOTO; Yoshimi SHINGAI; Miyuki INAI; Mitsuhiro NAKAMURA; Shoji IMAI

doi:10.1271/bbb.110837

Organic Chemistry Regular Papers

Radical Scavenging Activity of Spring Mountain Herbs in the Shikoku Mountain Area and Identification of Antiradical Constituents by Simple HPLC Detection and LC-MS Methods

Toshiya MASUDA, Tomoko INOUCHI, Aya FUJIMOTO, Yoshimi SHINGAI, Miyuki INAI, Mitsuhiro NAKAMURA, Shoji IMAI

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Keywords: mountain herb, radical scavenging activity, Polystichym ovato-paleaceum (Tsuyanashiinode), Sambucus racemosa subsp. sieboldiana (Niwatoko)

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2012 Volume 76 Issue 4 Pages 705-711

DOI https://doi.org/10.1271/bbb.110837

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Abstract

The functionality of spring mountain herbs, which were collected in the Kajigamori mountain area of Shikoku area in Japan, was investigated in the course of our studies for utilizing local plant resources. The radical scavenging activity of the extracts from seventeen herbs was measured. Among these herbs, two extracts from Polystichym ovato-paleaceum (Japanese name: Tsuyanashiinode) and Sambucus racemosa subsp. sieboldiana (Japanese name: Niwatoko) showed potent DPPH radical scavenging activity. The material evidence for the potent activity of the extracts was studied by a combination of our developed method for detecting antiradical compounds, LC-MS/MS, and enzymatic hydrolysis.

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