Acrolein, an α,β-Unsaturated Carbonyl, Inhibits Both Growth and PSII Activity in the Cyanobacterium Synechocystis sp. PCC 6803

Ginga SHIMAKAWA; Tatsuya IWAMOTO; Tomohito MABUCHI; Ryota SAITO; Hiroshi YAMAMOTO; Katsumi AMAKO; Toshio SUGIMOTO; Amane MAKINO; Chikahiro MIYAKE

doi:10.1271/bbb.130186

Environmental Science Regular Paper

Acrolein, an α,β-Unsaturated Carbonyl, Inhibits Both Growth and PSII Activity in the Cyanobacterium Synechocystis sp. PCC 6803

Ginga SHIMAKAWA, Tatsuya IWAMOTO, Tomohito MABUCHI, Ryota SAITO, Hiroshi YAMAMOTO, Katsumi AMAKO, Toshio SUGIMOTO, Amane MAKINO, Chikahiro MIYAKE

Author information

Ginga SHIMAKAWA
Department of Biological and Environmental Sciences, Faculty of Agriculture, Graduate School of Agricultural Science, Kobe University
Tatsuya IWAMOTO
Department of Biological and Environmental Sciences, Faculty of Agriculture, Graduate School of Agricultural Science, Kobe University
Tomohito MABUCHI
Department of Biological and Environmental Sciences, Faculty of Agriculture, Graduate School of Agricultural Science, Kobe University
Ryota SAITO
Department of Biological and Environmental Sciences, Faculty of Agriculture, Graduate School of Agricultural Science, Kobe University
Hiroshi YAMAMOTO
Department of Botany, Graduate School of Science, Kyoto University
Katsumi AMAKO
Faculty of Nutrition, Kobe-Gakuin University
Toshio SUGIMOTO
Department of Biological and Environmental Sciences, Faculty of Agriculture, Graduate School of Agricultural Science, Kobe University
Amane MAKINO
Department of Agriculture, Graduate School of Agricultural Science, Tohoku University
CREST, JST
Chikahiro MIYAKE
Department of Biological and Environmental Sciences, Faculty of Agriculture, Graduate School of Agricultural Science, Kobe University
CREST, JST

Keywords: acrolein, NADPH-dependent acrolein reduction (NAR), photosynthesis, plant diabetes, reactive carbonyls (RCs)

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Supplementary material

2013 Volume 77 Issue 8 Pages 1655-1660

DOI https://doi.org/10.1271/bbb.130186

View "Advance Publication" version (August 7, 2013).

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Abstract

In this study, we sought to determine whether and how an α,β-unsaturated carbonyl, acrolein, can inhibit the growth of the cyanobacterium Synechocystis sp. PCC6803 (S. 6803). Treatment of S. 6803 with 200 µM acrolein for 3 d significantly and irreversibly inhibited its growth. To elucidate the inhibitory mechanism, we examined the effects of acrolein on photosynthesis. In contrast to dark conditions, the addition of acrolein to S. 6803 under conditions of illumination lowered the CO₂-dependent O₂ evolution rate (photosynthetic activity). Furthermore, treatment with acrolein lowered the activity reducing dimethyl benzoquinone in photosystem II (PSII). Acrolein also suppressed the reduction rate for the oxidized form of the reaction center chlorophyll of photosystem I (PSI), P700. These results indicate that acrolein inhibited PSII activity in thylakoid membranes. The addition of 200 µM acrolein to the illuminated S. 6803 cells gradually increased the steady-state level (Fs) of Chl fluorescence and decreased the quantum yield of PSII. These results suggested that acrolein damaged the acceptor side of PSII. On the other hand, acrolein did not inhibit respiration. From the above results, we gained insight into the metabolism of acrolein and its physiological effects in S. 6803.

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