Formation and Some Propertes of Diacetyl Reductase From Klebsiella pneumoniae
1977 Volume 41 Issue 3 Pages 527-532
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1977 Volume 41 Issue 3 Pages 527-532
Formation and properties of diacetyl reductase were studied. Gluconate, acetate, pyruvate lactate, citrate and succinate increased the enzyme formation. The initial pH of the medium favorable for the enzyme formation was 7.0_??_8.0.
The enzyme was partially purified about 65-fold. The optimum pH value for the activity was 7.5 and the optimum temperature was 37°C. The enzyme was stable in the pH range of 7.0 to 8.0 and at the temperatures of 37°C and below. No metal ion which enhanced the enzyme activity was observed. Tris-HCl buffer reduced the enzyme activity.
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