ELISA Detection of Kudoa septempunctata in Raw Paralichthys olivaceus (Olive Flounder) using a Chicken Anti-Kudoa Antiserum

YUTAKA KIKUCHI; TAKAHIRO OHNISHI; HIROKO FURUSAWA; TAKAO KAWAI; YUTAKA FUKUDA; HIROSHI YOKOYAMA; YOSHIKO SUGITA-KONISHI

doi:10.4265/bio.18.193

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ELISA Detection of Kudoa septempunctata in Raw Paralichthys olivaceus (Olive Flounder) using a Chicken Anti-Kudoa Antiserum

YUTAKA KIKUCHI, TAKAHIRO OHNISHI, HIROKO FURUSAWA, TAKAO KAWAI, YUTAKA FUKUDA, HIROSHI YOKOYAMA, YOSHIKO SUGITA-KONISHI

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Keywords: Kudoa septempunctata, Paralichthys olivaceus, Chicken serum, ELISA, Immunohistochemical staining

JOURNAL FREE ACCESS

2013 Volume 18 Issue 4 Pages 193-197

DOI https://doi.org/10.4265/bio.18.193

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Abstract

Kudoa septempunctata is the causative agent of a foodborne disease associated with the consumption of raw Paralichthys olivaceus (olive flounder). Chickens were used to establish specific antibodies against K. septempunctata spores. A specific antiserum, CS#3, raised against sonicated spores, also recognized intact spores. The CS#3 antiserum showed high titers for sonicated and intact K. septempunctata spores and was suitable for both ELISA and immunohistochemical staining. Using homogenated raw olive flounder meat, the ELISA system detected more than 5.0×10⁵ spores in 1 g of tissue, which was consistent with the number determined by microscopic examination. The preparation of rapid detection kits for K. septempunctata spores in P. olivaceus muscle tissue using immunochromatography with CS#3 antiserum should be useful for preventing the foodborne disease in the field.

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