Nucleotide Sequence of the Gene Encoding NADH Dehydrogenase from an Alkalophile, Bacillus sp. Strain YN-1

Xuemin Xu; Noriyuki Koyama; Meizhen Cui; Akihiko Yamagishi; Yoshiaki Nosoh; Tairo Oshim

Nucleotide Sequence of the Gene Encoding NADH Dehydrogenase from an Alkalophile, Bacillus sp. Strain YN-1

Xuemin Xu, Noriyuki Koyama, Meizhen Cui, Akihiko Yamagishi, Yoshiaki Nosoh, Tairo Oshim

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JOURNAL FREE ACCESS

1991 Volume 109 Issue 5 Pages 678-683

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Abstract

The gene encoding NADH dehydrogenase from an alkalophile, Bacillus sp., was cloned and sequenced. The cloned DNA fragment contained an open reading frame of 1, 557 nucleotides which encodes a polypeptide composed of 519 amino acid residues (M_r 55, 830). The predicted amino acid sequence was consistent with the partial amino acid sequences including the N-terminal and C-terminal sequences determined in a previous study. Sequence comparison with other flavoenzymes revealed high homology between the present dehydrogenase and Escherichia coli thioredoxin reductase.

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