Human UDP-Galactose Translocator: Molecular Cloning of a Complementary DNA That Complements the Genetic Defect of a Mutant Cell Line Deficient in UDP-Galactose Translocator

Nobuhiko Miura; Nobuhiro Ishida; Masato Hoshino; Masatake Yamauchi; Takahiko Hara; Dai Ayusawa; Masao Kawakita

Nobuhiko Miura, Nobuhiro Ishida, Masato Hoshino, Masatake Yamauchi, Takahiko Hara, Dai Ayusawa, Masao Kawakita

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Keywords: CDC91, Golgi apparatus, UDP-galactose, UDP-galactose translocator, ZK370.7

JOURNAL FREE ACCESS

1996 Volume 120 Issue 2 Pages 236-241

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Abstract

We have cloned a cDNA that codes for a putative human UDP-galactose translocator (UGT) protein. The cDNA contained an open reading frame of 1, 179 base pairs encoding a novel protein of 393 amino acids. Introduction of the open reading frame sequence into a UGT-deficient mouse cell line, Had-1, complemented the genetic defect of the mutant, namely the inability to transport UDP-galactose from the cytosol to the Golgi lumen, as judged from the lectin-sensitivity spectrum of the transformant. To our knowledge, this is the first mammalian nucleotide-sugar translocator whose cDNA sequence has been described.

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