Granule formation of elongator-specific methionine transfer RNA caused by X-ray irradiation in a non-small lung carcinoma cell line (NCI-H1299)

Atsuto Katano; Ryu Miyagawa; Reiko Kobayashi; Hideomi Yamashita; Teppei Morikawa; Akira Sakumi; Yuki Tamari; Masashi Fukayama; Kuni Ohtomo; Keiichi Nakagawa

doi:10.11169/bioimages.25.1

Regular Article

Granule formation of elongator-specific methionine transfer RNA caused by X-ray irradiation in a non-small lung carcinoma cell line (NCI-H1299)

Atsuto Katano, Ryu Miyagawa , Reiko Kobayashi, Hideomi Yamashita, Teppei Morikawa, Akira Sakumi, Yuki Tamari, Masashi Fukayama, Kuni Ohtomo, Keiichi Nakagawa

Author information

Atsuto Katano
Department of Radiology, the University of Tokyo Hospital
Ryu Miyagawa Corresponding author
Department of Pathology, the University of Tokyo Hospital
Reiko Kobayashi
Department of Radiology, the University of Tokyo Hospital
Hideomi Yamashita
Department of Radiology, the University of Tokyo Hospital
Teppei Morikawa
Department of Pathology, the University of Tokyo Hospital
Akira Sakumi
Department of Radiology, the University of Tokyo Hospital
Yuki Tamari
Department of Radiology, the University of Tokyo Hospital
Masashi Fukayama
Department of Pathology, the University of Tokyo Hospital
Kuni Ohtomo
Department of Radiology, the University of Tokyo Hospital
Keiichi Nakagawa
Department of Radiology, the University of Tokyo Hospital

Keywords: tRNA, stress, granule, TIA-1, X-ray, irradiation

JOURNAL FREE ACCESS

2017 Volume 25 Pages 1-6

DOI https://doi.org/10.11169/bioimages.25.1

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Abstract

We herein report the subcellular distribution of methionine transfer RNA (tRNA^Met), which has two forms of initiator tRNA^Met (Meti) and elongator tRNA^Met (Mete). Meti induces translation initiation, and Mete is responsible for the elongation of the peptide chain. A recent report showed that the formation of tRNA^Met granules occurs following heat shock stress. We analyzed the subcellular distribution change in tRNA^Met using ionizing irradiation. The images acquired using confocal microscopy showed the presence of Mete granules—but not Meti granules—in the cytoplasm. Furthermore, while the Mete granules co-localized with T-cell intracellular antigen-1, they did not co-localize to typical stress granules nor processing body specific markers.

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