Different distribution of Cav3.2 and Cav3.1 transcripts encoding T-type Ca2+ channels in the embryonic heart of mice

Einosuke Mizuta; Manabu Shirai; Keita Arakawa; Kyoko Hidaka; Junichiro Miake; Haruaki Ninomiya; Masahiko Kato; Chiaki Shigemasa; Yasuaki Shirayoshi; Ichiro Hisatome; Takayuki Morisaki

doi:10.2220/biomedres.31.301

Full Papers

Different distribution of Cav3.2 and Cav3.1 transcripts encoding T-type Ca²⁺ channels in the embryonic heart of mice

Einosuke Mizuta, Manabu Shirai, Keita Arakawa, Kyoko Hidaka, Junichiro Miake, Haruaki Ninomiya, Masahiko Kato, Chiaki Shigemasa, Yasuaki Shirayoshi, Ichiro Hisatome, Takayuki Morisaki

Author information

Einosuke Mizuta
Division of Molecular Medicine and Therapeutics, Department of Multidisciplinary Internal Medicine, Tottori University Faculty of Medicine
Manabu Shirai
Department of Bioscience, National Cardiovascular Center Research Institute
Keita Arakawa
Department of Regenerative Medicine and Therapeutics, Institute of Regenerative Medicine and Biofunction, Tottori University Graduate School of Medical Science
Kyoko Hidaka
Department of Bioscience, National Cardiovascular Center Research Institute
Junichiro Miake
Division of Molecular Medicine and Therapeutics, Department of Multidisciplinary Internal Medicine, Tottori University Faculty of Medicine
Haruaki Ninomiya
Department of Biological Regulation, Tottori University Faculty of Medicine
Masahiko Kato
Division of Molecular Medicine and Therapeutics, Department of Multidisciplinary Internal Medicine, Tottori University Faculty of Medicine
Chiaki Shigemasa
Division of Molecular Medicine and Therapeutics, Department of Multidisciplinary Internal Medicine, Tottori University Faculty of Medicine
Yasuaki Shirayoshi
Department of Regenerative Medicine and Therapeutics, Institute of Regenerative Medicine and Biofunction, Tottori University Graduate School of Medical Science
Ichiro Hisatome
Department of Regenerative Medicine and Therapeutics, Institute of Regenerative Medicine and Biofunction, Tottori University Graduate School of Medical Science
Takayuki Morisaki
Department of Bioscience, National Cardiovascular Center Research Institute

JOURNAL FREE ACCESS

2010 Volume 31 Issue 5 Pages 301-305

DOI https://doi.org/10.2220/biomedres.31.301

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Abstract

We investigated the distribution of T-type Ca²⁺ channel mRNAs in the mouse embryonic heart. Cav3.2, but not Cav3.1, was expressed in the E8.5 embryonic heart along with cardiac progenitor markers (Nkx2.5, Tbx5, Isl-1) and contractile proteins (alpha and beta MHC). In the E10.5 heart, the distribution of Cav3.1 mRNA was confirmed in the AV-canal and overlapped with that of MinK or Tbx2. Cav3.2 mRNA was observed not only in the AV-canal but also in the outflow tract, along with MinK and Isl-1, indicating the expression of Cav3.2 in the secondary heart field. Thus, Cav3.2 may contribute to the development of the outflow tract from the secondary heart field in the embryonic heart, whereas Cav3.1 may be involved in the development of the cardiac conduction-system together with Cav3.2.

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