Strong Antihyperglycemic Effects of Water-Soluble Fraction of Brazilian Propolis and Its Bioactive Constituent, 3,4,5-Tri-O-caffeoylquinic Acid

Toshiro Matsui; Sumi Ebuchi; Tomoko Fujise; Kanthi J. M. Abesundara; Shima Doi; Hideo Yamada; Kiyoshi Matsumoto

doi:10.1248/bpb.27.1797

Regular Articles

Strong Antihyperglycemic Effects of Water-Soluble Fraction of Brazilian Propolis and Its Bioactive Constituent, 3,4,5-Tri-O-caffeoylquinic Acid

Toshiro Matsui, Sumi Ebuchi, Tomoko Fujise, Kanthi J. M. Abesundara, Shima Doi, Hideo Yamada, Kiyoshi Matsumoto

Author information

Toshiro Matsui
Department of Bioscience and Biotechnology, Division of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Graduate School, Kyushu University
Sumi Ebuchi
Department of Bioscience and Biotechnology, Division of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Graduate School, Kyushu University
Tomoko Fujise
Department of Bioscience and Biotechnology, Division of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Graduate School, Kyushu University
Kanthi J. M. Abesundara
Department of Bioscience and Biotechnology, Division of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Graduate School, Kyushu University
Shima Doi
Yamada Apiculture Center, Inc.
Hideo Yamada
Yamada Apiculture Center, Inc.
Kiyoshi Matsumoto
Department of Bioscience and Biotechnology, Division of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Graduate School, Kyushu University

Keywords: α-glucosidase, caffeoylquinic acid, propolis, antihyperglycemic effect, diabetes

JOURNAL FREE ACCESS

2004 Volume 27 Issue 11 Pages 1797-1803

DOI https://doi.org/10.1248/bpb.27.1797

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Abstract

To clarify the suppression of postprandial blood glucose rise via α-glucosidase (AGH) inhibitory action by natural compounds, propolis was examined in this study. A single oral administration of propolis extract (50% methanol fraction on XAD-2 column chromatography) in Sprague–Dawley rats demonstrated a potent antihyperglycemic effect with the significant AUC_{0—120 min} reduction of 38% at a dose of 20 mg/kg compared to that of controls. Among the active compounds isolated from the fraction, 3,4,5-tri-caffeoylquinic acid was found to be a prominent candidate that exerts the effect and shows a strong maltase-specific inhibition with an IC₅₀ value of 24 μM. In addition, the noncompetitive inhibition power apparently increased with the number of caffeoyl groups bound to quinic acid.

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