Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
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Effect of Vanadate on ATP-Induced Increase in Intracellular Calcium Ion Levels in Human Umbilical Vein Endothelial Cells
Namie Nejime, Yukari Tada, Satomi Kagota, Yoko Kubota, Ikuo Shibuichi, Yuki Shinoda, Tomohiro Yamamoto, Yasuo Watanabe, Kazumasa Shinozuka
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Keywords: vanadium, ATP, P2Y receptor, human umbilical vein endothelial cell, intracellular calcium ion
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2010 Volume 33 Issue 6 Pages 1060-1062

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Abstract

We investigated the effect of ammonium vanadate (vanadate) on ATP-induced increases in intracellular calcium ion level ([Ca2+]i) of human umbilical vein endothelial cells (HUVEC) by fluorescence confocal microscopic imaging using the Ca2+-sensitive probe Calcium Green 1/AM. The ATP analogue 2-methylthio-ATP (2meS-ATP), at 10 μM, significantly increased the [Ca2+]i of HUVEC, and this was abolished by 1 μM thapsigargin (a calcium pump inhibitor), whereas extracellular free calcium had no effect. Vanadate at 10 μM also significantly increased the [Ca2+]i of HUVEC, which was abolished by 1 μM thapsigargin. However, vanadate at 1 μM did not exert such a significant effect. We thus examined the influence of ≤1 μM vanadate for 24 h on 2meS-ATP-induced increase in [Ca2+]i. Vanadate significantly reduced the action of 2meS-ATP at 1 μM but not at 0.1 μM. Endogenously released ATP is known to induce various actions on endothelial cells. The present results suggest that vanadate exerts a regulatory influence on the function of vascular endothelial cells.

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© 2010 The Pharmaceutical Society of Japan
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