Effect of Methanol on the Frequency of Respiration-Deficient Mutation Induced by Ethidium Bromide in Yeast under Growing and Non-growing Conditions

HIROSHI HAMADA; MIYUKI YAMASHITA; NOBUHIRO KASHIGE; MISAO KOJIMA; TAMOTSU MORITA

doi:10.1248/cpb.36.4865

HIROSHI HAMADA, MIYUKI YAMASHITA, NOBUHIRO KASHIGE, MISAO KOJIMA, TAMOTSU MORITA

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Keywords: yeast, Saccharomyces cerevisiae, ethidium bromide, respiration-deficient mutation, methanol effect, growing condition, non-growing condition

JOURNAL FREE ACCESS

1988 Volume 36 Issue 12 Pages 4865-4870

DOI https://doi.org/10.1248/cpb.36.4865

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Abstract

The effect of methanol on the frequency of cytoplasmic respiration-deficient (RD) mutation induced by ethidium bromide was investigated in Saccharomyces cerevisiae. When growing cells were treated with ethidium bromide at concentrations higher than 1.0μg/ml, 95 to 100% of surviving cells were RD mutants as judged by the tetrazolium overlay method. The RD mutants induced by 1.0μg/ml ethidium bromide were drastically decreased by addition of 6-8% methanol in both growing and non-growing conditions. Methanol reduced uptake of ethidium bromide by yeast cells, but it did not modify the inhibitory effect of ethidium bromide on in vitro yeast mitochondrial deoxyribonucleic acid synthesis.

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